In this podcast, Director of GLP bioanalysis Lan Li reviews immunoassays versus mass spectrometry-based methods as tools for protein biomarker quantitation. Focusing specifically on mass spectrometry approaches, Lan explores various methodologies, such as top-down, middle-down and bottom-up, as well as the sample preparation procedures required. Lan concludes by reviewing the current challenges impacting protein biomarker quantification and how QPS Holdings, LLC is developing solutions to overcome them.

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LC–MS/MS for quantitation of protein biomarkers

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